Gene Synthesis Over 5 kb
Sequences over 5 kb require specialist providers or multi-fragment assembly. Very few providers offer direct synthesis at this length. Here is a comparison for a representative 8000 bp sequence.
| Provider | Product | Price (8,000 bp) | Per bp | Turnaround | Length range | Feasibility |
|---|---|---|---|---|---|---|
| ENFINIA plasmid DNA | $960.00 | $0.120 | 6–8 business days | 1,000–15,000 bp | Yes | |
| Gene in vector (≤500 bp) | — | Flat fee | 5–6 business days | 100–500 bp | Outside range | |
| GeneStrands fragment (≤500 bp) | — | Flat fee | 2–4 business days | 100–500 bp | Outside range | |
| GeneStrands fragment (501–1000 bp) | — | Flat fee | 3–5 business days | 501–1,000 bp | Outside range | |
| GeneStrands fragment (1001–2000 bp) | — | Flat fee | 5–8 business days | 1,001–2,000 bp | Outside range | |
| Gene fragment | — | $0.070 | 2–4 business days | 300–5,000 bp | Outside range | |
| gBlocks gene fragment | — | $0.070 | 1–3 business days | 125–3,000 bp | Outside range | |
| eBlocks gene fragment | — | $0.070 | 1–3 business days | 300–1,500 bp | Outside range | |
| ENFINIA linear DNA | — | $0.070 | 6–8 business days | 1,000–7,000 bp | Outside range | |
| Clonal gene | — | $0.090 | 4–10 business days | 300–5,000 bp | Outside range | |
| Gene synthesis (standard) | — | $0.180 | 12–16 business days | 500–3,000 bp | Outside range | |
| Gene in vector (501–3000 bp) | — | $0.200 | 15–20 business days | 501–3,000 bp | Outside range | |
| Gene synthesis (clonal) | — | $0.400 | 6–13 business days | 501–2,000 bp | Outside range |
Prices based on published list prices for a 8,000 bp sequence. Actual quotes may vary based on sequence complexity, volume discounts, and promotions. Contact vendors directly for exact pricing.
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Compare your sequenceFrequently asked questions
Who can synthesise genes over 5 kb?
Elegen supports linear DNA up to 7 kb and plasmid DNA up to 15 kb. For longer constructs, you may need to order multiple fragments and assemble them in your lab using Gibson Assembly or Golden Gate cloning.
What is the most cost-effective approach for very long genes?
For genes over 5 kb, ordering multiple overlapping fragments from a low-cost provider (Twist or IDT at $0.07/bp) and assembling them yourself is often cheaper than paying a single provider for end-to-end long gene synthesis.